Rabbit Anti-Human VEGFR2 (FLK1/KDR) (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35968 |
| Gene Id | 3791 |
| Gene Symbols | KDR |
| Protein Name / Synonyms | VEGFR2 (FLK1/KDR) (C-term) |
| Clonality | Polyclonal |
| Preparation | This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1356 amino acids from the C-terminal region of human VEGFR2 (FLK1/KDR). |
| Concentration (lot specific) | 1.270 mg/ml |
| Recommended Applications | Western Blotting, IF, FC, IHC-P |
| Research Area | Angiogenesis, Cancer, Autophagy, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Specificity
Other Names
KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; Fetal liver kinase 1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; CD_antigen=CD309; Flags: Precursor
NCBI Accession #
NP_002244.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
151527
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; FC: 1:10-50; IHC: 1:50-100
 | Western blot analysis of anti-KDR/FLK1 Pab (Cat# 102-17506) in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term)(Cat# 102-17506) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | VEGFR2 (FLK1/KDR) Antibody (C-term) (Cat# 102-17506) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.