Villin antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 7429 |
| Protein Name / Synonyms | Villin |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
D2S1471 antibody, VIL antibody, VIL1 antibody, villin-1 antibody, villin 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
93 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 7 and 246 of Villin
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin(107-10884) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Villin antibody detects VIL1 protein by Western blot analysis.
A. 50 µg mouse kidney lysate/extract
7.5 % SDS-PAGE
Villin antibody (107-10884) dilution: 1:1000
A. 50 µg mouse kidney lysate/extract
7.5 % SDS-PAGE
Villin antibody (107-10884) dilution: 1:1000
Villin antibody detects VIL1 protein by Western blot analysis.
A. 50 µg rat kidney lysate/extract
7.5 % SDS-PAGE
Villin antibody (107-10884) dilution: 1:2000
A. 50 µg rat kidney lysate/extract
7.5 % SDS-PAGE
Villin antibody (107-10884) dilution: 1:2000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Villin (107-10884) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10884 diluted at 1:5000
A: Hep G2
7.5% SDS PAGE
107-10884 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.