Rabbit Anti-Human VIME Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08670 |
| Gene Id | 7431 |
| Gene Symbols | VIM |
| Protein Name / Synonyms | VIME |
| Clonality | Polyclonal |
| Preparation | This Vimentin antibody is generated from rabbits immunized with Vimentin recombinant protein. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.
Specificity
Other Names
VIM; Vimentin
NCBI Accession #
NP_003371.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
53652
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of VIME Antibody (Cat# 102-17991) in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab. |
 | VIME Antibody (Cat# 102-17991)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated. |
 | VIME Antibody (Cat# 102-17991) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.