VPS35 antibody [C3], C-Terminus, 0.93 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 55737 |
| Protein Name / Synonyms | VPS35, C-term |
| Concentration (lot specific) | 0.93 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp434E1211 antibody, DKFZp434P1672 antibody, FLJ10752 antibody, FLJ13588 antibody, FLJ20388 antibody, MEM3 antibody, VPS35 antibody, maternal-embryonic 3 antibody, hVPS35 antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
92 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID# Q96QK1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HCT116, using VPS35(107-10729) antibody at 1:500 dilution.
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (107-10729) dilution: 1:250.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (107-10729) dilution: 1:250.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10729 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-10729 diluted at 1:1000
VPS35 antibody detects VPS35 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
VPS35 antibody (107-10729) dilution: 1:500
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
VPS35 antibody (107-10729) dilution: 1:500
Sample(30 µg of whole cell lysate)
A:H1299
B:HeLa S3
C:Hep G2
D:MOLT4
E:Raji
7.5% SDS PAGE
107-10729 diluted at 1:500
A:H1299
B:HeLa S3
C:Hep G2
D:MOLT4
E:Raji
7.5% SDS PAGE
107-10729 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.