WBSCR22 antibody, 0.42 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 114049 |
| Protein Name / Synonyms | WBSCR22 |
| Concentration (lot specific) | 0.42 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ44236 antibody, HASJ4442 antibody, HUSSY-3 antibody, MGC19709 antibody, MGC2022 antibody, MGC5140 antibody, PP3381 antibody, WBMT antibody, WBSCR22 antibody, Williams-Beuren candidate region putative methyltransferase antibody, Williams-Beuren syndrome chromosomal region 22 protein antibody, uncharacterized methyltransferase WBSCR22 antibody, Williams Beuren syndrome chromosome region 22 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 263 of WBSCR22 (Uniprot ID# O43709)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, IP: 1:500-1:1000.ICC/IF:Use at an dependent assay. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
107-10563 diluted at 1:1000
A: HeLa
B: HCT116
12% SDS PAGE
107-10563 diluted at 1:1000
WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 µg of WBSCR22 antibody (107-10563) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (107-10563) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (107-10563) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.