WNT11 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7481 |
| Protein Name / Synonyms | WNT11 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HWNT11 antibody, MGC141946 antibody, MGC141948 antibody, WNT11 antibody, protein Wnt-11 antibody, "wingless-type MMTV integration site family, member 11 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
39 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID# O96014)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
WNT11 antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: WNT11 protein stained by WNT11 antibody (107-10576) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: WNT11 protein stained by WNT11 antibody (107-10576) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
WNT11 antibody (107-10576) dilution: 1:500.
Sample: Paraffin-embedded mouse colon.
WNT11 antibody (107-10576) dilution: 1:500.
WNT11 antibody detects WNT11 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
12 % SDS-PAGE
WNT11 antibody (107-10576) dilution: 1:1000
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
12 % SDS-PAGE
WNT11 antibody (107-10576) dilution: 1:1000
WNT11 antibody detects WNT11 protein by western blot analysis.
A. 50 µg mouse heart extract
10 % SDS-PAGE
WNT11 antibody (107-10576) dilution: 1:1000
A. 50 µg mouse heart extract
10 % SDS-PAGE
WNT11 antibody (107-10576) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11(107-10576) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11(107-10576) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.