XRCC4 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 7518 |
| Protein Name / Synonyms | XRCC4 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
XRCC4 antibody, X-ray repair cross-complementing protein 4 antibody, DNA repair protein XRCC4 antibody, X-ray repair, complementing defective, repair in Chinese hamster antibody, X-ray repair complementing defective repair in Chinese hamster cells 4 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 216 and 309 of XRCC4 (Uniprot ID# Q13426)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (107-10858) dilution: 1:500.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (107-10858) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
C: HL-60
10% SDS PAGE
107-10858 diluted at 1:1000
A: Jurkat
B: K562
C: HL-60
10% SDS PAGE
107-10858 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4(107-10858) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.