Rabbit Anti-Human YBX1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P67809 |
| Gene Id | 4904 |
| Gene Symbols | YBX1 |
| Protein Name / Synonyms | YBX1 (Center) |
| Clonality | Polyclonal |
| Preparation | This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-178 amino acids from the Central region of human YBX1. |
| Concentration (lot specific) | 0.310 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Rabbit
Other Names
YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; DNA-binding protein B; Enhancer factor I subunit A; Y-box transcription factor; Y-box-binding protein 1
NCBI Accession #
NP_004550.2
Other Accession #
P62961; Q28618; P62960; P67808; NP_004550.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35924
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | YBX1 Antibody (Center) (Cat# 102-14493) western blot analysis in 293T cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow). |
 | Fluorescent image of A549 cell stained with YBX1 Antibody (Center)(Cat# 102-14493/SA120807BH).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). YBX1 immunoreactivity is localized to Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.