Synonyms
YKL-39 antibody, YKL39 antibody, CHI3L2 antibody, chitinase-3-like protein 2 antibody, chondrocyte protein 39 antibody, chitinase 3-like 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 27 and 374 of YKL-39 (Uniprot ID# Q15782)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CHI3L2(107-10433) antibody at 1:500 dilution.

YKL-39 antibody [N1C3] detects YKL-39 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole extract of human CHI3L2-transfected 293T cells
10 % SDS-PAGE
YKL-39 antibody [N1C3] (107-10433) dilution: 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.