Rabbit Anti-Human YWHAB (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P31946 |
| Gene Id | 7529 |
| Gene Symbols | YWHAB |
| Protein Name / Synonyms | YWHAB (C-term) |
| Clonality | Polyclonal |
| Preparation | This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human YWHAB. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | IHC-P, Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Monkey
Other Names
YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; 14-3-3 protein beta/alpha, N-terminally processed
NCBI Accession #
NP_003395.1;NP_647539.1
Other Accession #
Q5PRD0; P35213; Q9CQV8; Q4R572; Q5ZLQ6; P68250
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28082
Recommended Dilutions
IHC: 1:50-100; WB: 1:1000; IF: 1:10-50
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | The anti-YWHAB Pab (Cat# 102-17838) is used in Western blot to detect YWHAB in Jurkat cell lysate. |
 | Western blot analysis of YWHAB (arrow) using rabbit polyclonal YWHAB Antibody (C-term) (Cat# 102-17838). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene. |
 | Confocal immunofluorescent analysis of YWHAB Antibody (C-term)(Cat# 102-17838) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.