Synonyms
C9orf60 antibody, Hzyg antibody, RP11-545E17.4 antibody, ZYG antibody, ZYG11BL antibody, ZER1 antibody, protein zer-1 homolog antibody, zyg-11 homolog B-like protein antibody, homolog of Zyg-11 antibody, ZYG homolog antibody, zer-1 homolog (C. elegans) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
88 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 537 and 766 of ZER1 (Uniprot ID# Q7Z7L7)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ZER1(107-10658) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10658 diluted at 1:1000

Sample(30 ug whole cell lysate)
A:A431
B:H1299
7.5% SDS PAGE
107-10658 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.