Rabbit Anti-Human ZHX2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9Y6X8 |
| Gene Id | 22882 |
| Gene Symbols | ZHX2 |
| Protein Name / Synonyms | ZHX2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-131 amino acids from the N-terminal region of human ZHX2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.
Specificity
Predicted Reactivity
Mouse
Other Names
ZHX2; AFR1; KIAA0854; RAF; Zinc fingers and homeoboxes protein 2; Alpha-fetoprotein regulator 1; Regulator of AFP; Zinc finger and homeodomain protein 2
NCBI Accession #
NP_055758.1
Other Accession #
Q8C0C0; NP_055758.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
92307
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | ZHX2 Antibody (N-term)(Cat# 102-13995) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow). |
 | Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term)(Cat# 102-13995).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.