Synonyms
FLJ33906 antibody, KIAA0426 antibody, ZF47 antibody, ZFP306 antibody, ZNF306 antibody, ZNF309 antibody, ZSCAN13 antibody, Zfp47 antibody, dJ874C20.1 antibody, ZKSCAN3 antibody, zinc finger protein with KRAB and SCAN domains 3 antibody, zinc finger protein zfp47 antibody, zinc finger and SCAN domain-containing protein 13 antibody, zinc finger protein 306 antibody, zinc finger protein 47 homolog antibody, zinc finger protein 309 antibody, zinc finger with KRAB and SCAN domains 3 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 394 of ZKSCAN3 (Uniprot ID# Q9BRR0)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded normal lung, using ZKSCAN3(107-10581) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF306 (107-10581) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:HeLa S3
B:Hep G2
7.5% SDS PAGE
107-10581 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.