ZNF143 antibody, 0.83 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 7702 |
| Protein Name / Synonyms | ZNF143 |
| Concentration (lot specific) | 0.83 mg/ml |
| Recommended Applications | ICC/IF, Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
SBF antibody, STAF antibody, pHZ-1 antibody, ZNF143 antibody, selenocysteine tRNA gene transcription-activating factor antibody, SPH-binding factor antibody, hStaf antibody, transcriptional activator Staf antibody, zinc finger protein 143 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
69 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 409 and 621 of ZNF143 (Uniprot ID# P52747)
Applications
Applications Tested
ICC/IF,Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZNF143(107-10343) antibody at 1:500 dilution.
ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 µg of ZNF143 antibody 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10343 diluted at 1:1000
A: Hep G2
7.5% SDS PAGE
107-10343 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.