Synonyms
MGC110983 antibody, ZPR1 antibody, ZNF259 antibody, zinc finger protein ZPR1 antibody, zinc finger protein 259 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
51 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 159 and 459 of ZNF259 (Uniprot ID# O75312)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZNF259(107-10836) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ZNF259(107-10836) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10836 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.