ZO-1 antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 7082 |
| Protein Name / Synonyms | ZO-1, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
195 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 296 of ZO-1 (Uniprot ID# Q07157)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TJP1 protein stained by ZO-1 antibody [N1N2], N-term (107-10785) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TJP1 protein stained by ZO-1 antibody [N1N2], N-term (107-10785) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1(107-10785) antibody at 1:250 dilution.
Sample(30 ug whole cell lysate)
A:H1299
5% SDS PAGE
107-10785 diluted at 1:3000
A:H1299
5% SDS PAGE
107-10785 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.