Synonyms
FLJ93221 antibody, ZPBP1 antibody, ZPBP antibody, zona pellucida-binding protein 1 antibody, zona pellucida binding protein 1 antibody, zona pellucida binding protein antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 29 and 275 (Q9BS86) of ZPBP
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using ZPBP(107-10510) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:A431
B:HeLa S3
12% SDS PAGE
107-10510 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.