Rabbit Anti-ID1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P41134 |
| Gene Id | 3397 |
| Gene Symbols | ID1 |
| Protein Name / Synonyms | ID1 (Center) |
| Clonality | Polyclonal |
| Preparation | This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Specificity
Predicted Reactivity
Rat
Other Names
ID1; BHLHB24; ID; DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; Inhibitor of DNA binding 1
NCBI Accession #
NP_002156.2;NP_851998.1
Other Accession #
P41135; P20067; NP_851998.1; NP_002156.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
16133
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | ID1 Antibody (Center) (Cat# 102-11226) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow). |
 | ID1 Antibody (Center)(Cat# 102-11226) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow). |
 | ID1 Antibody (Center) (Cat# 102-11226)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of ID1 Antibody (Center)(Cat# 102-11226) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | ID1 Antibody (Center) (Cat# 102-11226) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.