IKBKE Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14164 |
| Gene Id | 9641 |
| Gene Symbols | IKBKE |
| Protein Name / Synonyms | IKBKE, IKK-E, IKK-i, IKKE, IKKI, inhibitor of nuclear factor kappa-B kinase subunit epsilon |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
70kDa/80kDa
Observed MW
93kDa
Immunogen
Recombinant protein of human IKBKE
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IKBKE antibody (144-00244) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using IKBKE antibody (144-00244) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using IKBKE antibody (144-00244) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using IKBKE antibody (144-00244) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug IKBKE antibody (144-00244). Western blot was performed from the immunoprecipitate using IKBKE antibody (144-00244) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.