IKBKG Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9Y6K9 |
| Gene Id | 8517 |
| Gene Symbols | IKBKG |
| Protein Name / Synonyms | IKBKG, AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, NF-kappa-B essential modulator |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
36kDa/48kDa/55kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKBKG (NP_003630.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IKBKG Antibody (144-12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] IKBKG Polyclonal Antibody (144-12536)](https://cdn.sanity.io/images/g51potcz/production/a1dcc9e40d8ecb65bd4c2b2ecf555974251cc0f6-307x350.jpg)
Western blot analysis of extracts from normal (control) and IKBKG knockout (KO) 293T cells, using IKBKG antibody (144-12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] IKBKG Polyclonal Antibody (144-12536)](https://www.raybiotech.com/files/images/Antibodies/144/144-12536/2.jpg)
Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKBKG antibody (144-12536). Western blot was performed from the immunoprecipitate using IKBKG antibody (144-12536) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (144-12536)](https://www.raybiotech.com/files/images/Antibodies/144/144-12536/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.