PATJ, Cipp, INADL, InaD-like, hINADL, inaD-like protein
Accession Number
Q8NI35
Gene Symbols
INADL
Gene Id
10207
Recommended Applications
Western Blotting,IHC
Purity
Affinity purification
Background
This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
125kDa/167kDa/170kDa/173kDa/196kDa
Observed MW
140kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 450-680 of human INADL (NP_795352.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using INADL antibody (144-08476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse kidney using INADL antibody (144-08476) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using INADL antibody (144-08476) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using INADL antibody (144-08476) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
