INTS6 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UL03 |
| Gene Id | 26512 |
| Gene Symbols | INTS6 |
| Protein Name / Synonyms | INTS6, DBI-1, DDX26, DDX26A, DICE1, HDB, INT6, Notchl2, integrator complex subunit 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
12kDa/98kDa/100kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human INTS6 (NP_036273.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using INTS6 antibody (144-06634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody (144-06634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using INTS6 Antibody (144-06634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using INTS6 Antibody (144-06634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody (144-06634) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using INTS6 antibody (144-06634). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.