[KO Validated] IRF3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14653 |
| Gene Id | 3661 |
| Gene Symbols | IRF3 |
| Protein Name / Synonyms | IRF3, IIAE7, interferon regulatory factor 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.01% thimerosal, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, ChIP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
ChIP 1:20 - 1:100
IHC 1:50 - 1:200
ChIP 1:20 - 1:100
Format
Calculated MW
12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IRF3 antibody (144-11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] IRF3 Polyclonal Antibody (144-11118)](https://cdn.sanity.io/images/g51potcz/production/51d1351254974597b799b75f08a642d300932ddb-387x350.jpg)
Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (144-11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] IRF3 Polyclonal Antibody (144-11118)](https://cdn.sanity.io/images/g51potcz/production/084d4cc0729d4bb93410f27a85afad058b4350a6-350x350.jpg)
Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (144-11118) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (144-11118)](https://cdn.sanity.io/images/g51potcz/production/6307b8e8fee449590aff40123640922d359651ac-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (144-11118) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (144-11118)](https://cdn.sanity.io/images/g51potcz/production/f57cd72663630c21bdaf4522501987f7b3e8a971-539x350.jpg)
Chromatin immunoprecipitation analysis extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (144-11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.![Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (144-11118)](https://www.raybiotech.com/files/images/Antibodies/144/144-11118/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.