ISG15 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P05161 |
| Gene Id | 9636 |
| Gene Symbols | ISG15 |
| Protein Name / Synonyms | ISG15, G1P2, IFI15, IMD38, IP17, UCRP, hUCRP, ubiquitin-like protein ISG15 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
17kDa
Observed MW
14kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ISG15 antibody (144-01182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using ISG15 Antibody (144-01182) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ISG15 Antibody (144-01182) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ISG15 Antibody (144-01182) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ISG15 Antibody (144-01182) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.