ITGA2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17301 |
| Gene Id | 3673 |
| Gene Symbols | ITGA2 |
| Protein Name / Synonyms | ITGA2, BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2, integrin alpha-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
129kDa
Observed MW
150kDa
Immunogen
Recombinant protein of human ITGA2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ITGA2 antibody (144-07629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)](https://cdn.sanity.io/images/g51potcz/production/274bf1665ec62f247c130803dc1540dd8a3a191e-327x350.jpg)
Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (144-07629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)](https://cdn.sanity.io/images/g51potcz/production/5b26048b0cab6ab7901f5be265e256b6dce203a8-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (144-07629) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)](https://cdn.sanity.io/images/g51potcz/production/60b62c890c2a8e1fbdd591b0725a70fbfffd075e-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (144-07629) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)](https://cdn.sanity.io/images/g51potcz/production/067689a1468ba1a73e931af7cb87eec2db941d2b-368x550.jpg)
Immunofluorescence analysis of U2OS cells using ITGA2 antibody (144-07629) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)](https://www.raybiotech.com/files/images/Antibodies/144/144-07629/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.