This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 797-1056 of human KIF11 (NP_004514.2).
Western blot analysis of extracts of various cell lines, using KIF11 antibody (144-07907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using KIF11 antibody (144-07907) at dilution of 1:100. Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.