KRT5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P13647 |
| Gene Id | 3852 |
| Gene Symbols | KRT5 |
| Protein Name / Synonyms | KRT5, CK5, DDD, DDD1, EBS2, K5, KRT5A, keratin 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
62kDa
Observed MW
70kDa
Immunogen
Recombinant protein of human KRT5
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KRT5 antibody (144-02662) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (144-02662) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (144-02662) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using KRT5 Antibody (144-02662) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KRT5 antibody (144-02662).
Expiration:
12 months from the date of shipment when stored properly.