The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
138kDa/139kDa/140kDa
Observed MW
200kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using L1CAM antibody (144-08555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (144-08555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.
![Western blot - [KO Validated] L1CAM Polyclonal Antibody (144-08555)](/files/images/Antibodies/144/144-08555/1.jpg "Western blot - [KO Validated] L1CAM Polyclonal Antibody (144-08555)")
![Western blot - [KO Validated] L1CAM Polyclonal Antibody (144-08555)](/files/images/Antibodies/144/144-08555/2.jpg "Western blot - [KO Validated] L1CAM Polyclonal Antibody (144-08555)")
