This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor involved in the control of differentiation and development of the pituitary gland. Mutations in this gene cause combined pituitary hormone deficiency 4.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
43kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 121-390 of human LHX4 (NP_203129.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LHX4 Antibody (144-08072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
