LMNB2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q03252 |
| Gene Id | 84823 |
| Gene Symbols | LMNB2 |
| Protein Name / Synonyms | LMNB2, EPM9, LAMB2, LMN2, lamin-B2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
69kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LMNB2 antibody (144-06483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody (144-06483) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody (144-06483) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using LMNB2 antibody (144-06483). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.