Rabbit Anti-LMO4 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P61968 |
| Gene Id | 8543 |
| Gene Symbols | LMO4 |
| Protein Name / Synonyms | LMO4 (Center) |
| Clonality | Polyclonal |
| Preparation | This LMO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human LMO4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | FC, IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene.
Specificity
Predicted Reactivity
Bovine
Other Names
LMO4; LIM domain transcription factor LMO4; Breast tumor autoantigen; LIM domain only protein 4
NCBI Accession #
NP_006760.1
Other Accession #
P61969; Q3SWZ8; NP_006760.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
17994
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent image of A549 cell stained with LMO4 Antibody (Center)(Cat# 102-10789).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).LMO4 immunoreactivity is localized to Nucleus significantly. |
 | LMO4 Antibody (Center) (Cat# 102-10789) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the LMO4 antibody detected the LMO4 protein (arrow). |
 | LMO4 Antibody (Center) (Cat# 102-10789) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.