Rabbit Anti-M Sirt3 (C-term) Antibody, 200 µl
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Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Hamster |
| Host Species | Rabbit |
| Accession Number | Q8R104 |
| Gene Id | 64384 |
| Gene Symbols | SIRT3 |
| Protein Name / Synonyms | M Sirt3 (C-term) |
| Clonality | Polyclonal |
| Preparation | This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3. |
| Concentration (lot specific) | 0.260 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Specificity
Other Names
Sirt3; Sir2l3; NAD-dependent protein deacetylase sirtuin-3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3
NCBI Accession #
NP_001120823.1;NP_001171275.1;NP_071878.2
Other Accession #
NP_071878.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28822
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody (C-term) (Cat# 102-16696). 102-16696 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
 | Western blot analysis of Sirt3 Antibody (C-term) (Cat# 102-16696) in mouse brain tissue lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab. |
 | Western blot analysis of Sirt3 Antibody (C-term) (Cat# 102-16696) in CHO cell line lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab. |
 | Sirt3 Antibody (C-term) (Cat# 102-16696) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
MOUSE
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.