MAD1L1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9Y6D9 |
| Gene Id | 8379 |
| Gene Symbols | MAD1L1 |
| Protein Name / Synonyms | MAD1L1, MAD1, PIG9, TP53I9, TXBP181, MAD1 mitotic arrest deficient like 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
72kDa/83kDa
Observed MW
95kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (144-01153) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (144-01153) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (144-01153) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (144-01153).
Expiration:
12 months from the date of shipment when stored properly.