MAP2K1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q02750 |
| Gene Id | 5604 |
| Gene Symbols | MAP2K1 |
| Protein Name / Synonyms | MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Dilutions
WB 1:1000 - 1:3000
Format
Calculated MW
40kDa/43kDa
Observed MW
45kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAP2K1 antibody (144-12687) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (144-12687)](https://cdn.sanity.io/images/g51potcz/production/fe6e04dbcd8837a47b358fe472ae9ee5eb48c4a0-609x350.jpg)
Western blot analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MAP2K1 antibody (144-12687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (144-12687)](https://www.raybiotech.com/files/images/Antibodies/144/144-12687/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.