MAPK9 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P45984 |
| Gene Id | 5601 |
| Gene Symbols | MAPK9 |
| Protein Name / Synonyms | MAPK9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK, mitogen-activated protein kinase 9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation, MAPK Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
27kDa/44kDa/48kDa
Observed MW
54kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAPK9 antibody (144-01251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)](https://cdn.sanity.io/images/g51potcz/production/02e283a1faf2b2d30b6485769149e7812f99efd1-497x350.jpg)
Western blot analysis of extracts from normal (control) and MAPK9 knockout (KO) 293T cells, using MAPK9 antibody (144-01251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)](https://cdn.sanity.io/images/g51potcz/production/e906a9d9587db7b219294e8b4f142ce4367c0894-350x350.jpg)
Immunohistochemistry of paraffin-embedded human breast cancer using MAPK9 antibody (144-01251) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)](https://cdn.sanity.io/images/g51potcz/production/0069e7d8b5319cad1e10f2b318eb1e26d2db8f11-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using MAPK9 antibody (144-01251) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)](https://cdn.sanity.io/images/g51potcz/production/2deb858a068369384ceba12553a3ed7180a459a9-366x550.jpg)
Immunofluorescence analysis of U2OS cells using MAPK9 antibody (144-01251). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)](https://www.raybiotech.com/files/images/Antibodies/144/144-01251/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.