This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MARS (NP_004981.2).
Western blot analysis of extracts of various cell lines, using MARS antibody (144-09938) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
12 months from the date of shipment when stored properly.