This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-151 amino acids from the N-terminal region of human MBD2.
Host Species
Rabbit
Clonality
Polyclonal
Species
Human, Mouse
Introduction
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Specificity
Other Names
MBD2; Methyl-CpG-binding domain protein 2; Demethylase; Methyl-CpG-binding protein MBD2
NCBI Accession #
NP_003918.1;NP_056647.1
Other Accession #
Q9Z2E1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43255
Recommended Dilutions
WB: 1:1000
The anti-MBD2 N-term Antibody (Cat# 102-10754) is used in Western blot to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate.
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
