This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
57kDa/61kDa
Observed MW
61kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MCCC2 antibody (144-07990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cells, using MCCC2 antibody (144-07990) at 1:1000 dilution ratio through one-step method. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using MCCC2 antibody (144-07990) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MCCC2 antibody (144-07990) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
