MCM3 antibody [N1N3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4172 |
| Protein Name / Synonyms | MCM3 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HCC5 antibody, MGC1157 antibody, P1-MCM3 antibody, P1.h antibody, RLFB antibody, MCM3 antibody, RLF subunit beta antibody, MCM3 minichromosome maintenance deficient 3 antibody, minichromosome maintenance deficient 3 antibody, DNA replication factor MCM3 antibody, DNA polymerase alpha holoenzyme-associated protein P1 antibody, p102 antibody, hRlf beta subunit antibody, "replication licensing factor, beta subunit antibody", cervical cancer proto-oncogene 5 antibody, DNA replication licensing factor MCM3 antibody, minichromosome maintenance complex component 3 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
91 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 42 and 302 of MCM3 (Uniprot ID# P25205)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(107-10953) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MCM3(107-10953) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10953 diluted at 1:10000
A: Hep G2
7.5% SDS PAGE
107-10953 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.