Rabbit Anti-MEK1 (MAP2K1) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q02750 |
| Gene Id | 5604 |
| Gene Symbols | MAP2K1 |
| Protein Name / Synonyms | MEK1 (MAP2K1) (N-term) |
| Clonality | Polyclonal |
| Preparation | This MEK1 (MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK1 (MAP2K1). |
| Concentration (lot specific) | 0.45 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Specificity
Predicted Reactivity
Rat, Rabbit
Other Names
MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1
NCBI Accession #
NP_002746.1
Other Accession #
Q01986; P29678; P31938
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43439
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of lysates from Jurkat,rat PC-12 and C6 cell line,mouse brain tissue (from left to right),using MAP2K1 Antibody (P1)(Cat# 102-17725).102-17725 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. |
 | Western blot analysis of lysates from Jurkat,rat PC-12,mouse NIH/3T3,rat C6 cell line and mouse brain tissue (from left to right),using MAP2K1 Antibody (P1)(Cat# 102-17725).102-17725 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.