Rabbit Anti-MEK2 (MAP2K2) (Center) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P36507 |
| Gene Id | 5605 |
| Gene Symbols | MAP2K2 |
| Protein Name / Synonyms | MEK2 (MAP2K2) (Center) |
| Clonality | Polyclonal |
| Preparation | This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human MEK2 (MAP2K2). |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Specificity
Other Names
MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2
NCBI Accession #
NP_109587.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
44424
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:50-100
 | Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center)(Cat# 102-17729/SA110819AW).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). MEK2 immunoreactivity is localized to Cytoplasm significantly. |
 | The anti-MAP2K2 Pab (Cat# 102-17729) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate. |
 | Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) (Cat# 102-17729).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | MAP2K2 Antibody (A278) (Cat# 102-17729) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.