Rabbit Anti-Metalloproteinase inhibitor 4( TIMP4) Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAH10553 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 4, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein TIMP4.The membrane blot was probed with anti-TIMP4 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein TIMP4 from E.coli cell (26kDa). |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Metalloproteinase inhibitor 4 (TIMP4) is an enzyme in humans that are involved in the regulation of platelet aggregation and recruitment and may play a role in hormonal regulation and endometrial tissue remodeling.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat TIMP4 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -86°C. After reconstitution, it can be aliquoted and stored
Related Products
RayBio® Human TIMP-4 ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine (Cat: ELH-TIMP4-001) Recombinant Human TIMP4 [from E. coli] (Cat: 230-00269)
References
- Olson TM, et al.Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and Timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively. Genomics 51 (1): 148151.
- Greene J, et al.Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4. J Biol Chem 271 (48): 3037530380.
Expiration:
12 months from the date of shipment when stored properly.
