METTL3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q86U44 |
| Gene Id | 56339 |
| Gene Symbols | METTL3 |
| Protein Name / Synonyms | METTL3, IME4, M6A, MT-A70, Spo8, hMETTL3, methyltransferase like 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
25kDa/64kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using METTL3 antibody (144-08370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody (144-08370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody (144-08370). Western blot was performed from the immunoprecipitate using METTL3 antibody (144-08370) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.