MMP14 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P50281 |
| Gene Id | 4323 |
| Gene Symbols | MMP14 |
| Protein Name / Synonyms | MMP14, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS, MT-MMP1, matrix metallopeptidase 14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
65kDa
Observed MW
46-70kDa
Immunogen
Recombinant protein of human MMP14
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MMP14 antibody (144-02549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using MMP14 antibody (144-02549) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MMP14 antibody (144-02549) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using MMP14 antibody (144-02549) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MMP14 antibody (144-02549) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.