Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues.
WB 1:500 - 1:2000
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AQP8 (NP_001160.2).
Western blot analysis of extracts of various cell lines, using AQP8 antibody (144-08539) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
12 months from the date of shipment when stored properly.