Rabbit Anti-Mouse EIF2S1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | P05198 |
| Gene Id | 1965 |
| Gene Symbols | EIF2S1 |
| Protein Name / Synonyms | EIF2S1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1. |
| Concentration (lot specific) | 0.310 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Specificity
Predicted Reactivity
Rat, Bovine, Chicken, Drosophila, Yeast
Other Names
EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha
NCBI Accession #
NP_004085.1
Other Accession #
P20459; P68101; Q6ZWX6; P41374; Q5ZLX2; P68102; NP_004085.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
36112
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50
 | EIF2S1 Antibody (N-term) (Cat# 102-12016) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF2S1 antibody detected the EIF2S1 protein (arrow). |
 | Confocal immunofluorescent analysis of EIF2S1 Antibody (N-term) (Cat# 102-12016) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
 | EIF2S1 Antibody (N-term) (Cat# 102-12016)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.