WB 1:500 - 1:2000
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human G0S2 (NP_056529.1).
Western blot analysis of extracts of various cell lines, using G0S2 Antibody (144-09970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
12 months from the date of shipment when stored properly.