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Product Description

Specifications

Size	400 µl
Protein Name / Synonyms	LIN28A (Center)
Accession Number	Q9H9Z2
Gene Symbols	LIN28A
Gene Id	79727
Concentration (lot specific)	2.0 mg/ml
Preparation	This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A.
Host Species	Rabbit
Clonality	Polyclonal
Species	Mouse

Introduction

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

Specificity

Other Names

LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1

NCBI Accession #

NP_078950.1

Other Accession #

NP_078950

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22743

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Immunofluorescent analysis of A549 cells, using LIN28A Antibody (Center) (Cat# 102-10989). 102-10989 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor^® 554 (red) conjugated Phalloidin (red).
	LIN28A Antibody (Center) (Cat# 102-10989) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).
	Fluorescent confocal image of SY5Y cells stained with 102-10989 LIN28A (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-10989 LIN28A (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
	LIN28A Antibody (Center) (Cat# 102-10989)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIN28A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	LIN28A Antibody (Center) (Cat# 102-10989) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.