This Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1606-1641 amino acids from the C-terminal region of mouse JMJD3.
Host Species
Rabbit
Clonality
Polyclonal
Species
Mouse
Introduction
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Specificity
Predicted Reactivity
Human
Other Names
Kdm6b; Jmjd3; Kiaa0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3
NCBI Accession #
NP_001017426.1
Other Accession #
O15054
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
176355
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
Western blot analysis of anti-JMJD3 C-term Pab (Cat# 102-10600) in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.
Formalin-fixed and paraffin-embedded human Skeletal Muscle tissue reacted with JMJD3 Antibody(C-term)(Cat# 102-10600), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Antigen Source
MOUSE
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
