Rabbit Anti-Mouse Prkca (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P20444 |
| Gene Id | 18750 |
| Gene Symbols | PRKCA |
| Protein Name / Synonyms | Prkca (N-term) |
| Clonality | Polyclonal |
| Preparation | This Mouse Prkca antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Prkca. |
| Concentration (lot specific) | 0.380 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4 (By similarity). PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Specificity
Predicted Reactivity
Rat
Other Names
Prkca; Pkca; Protein kinase C alpha type
NCBI Accession #
NP_035231.2
Other Accession #
P05696; NP_035231.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
76852
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:10-50
 | Mouse Prkca Antibody (N-term) (Cat# 102-12404) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow). |
 | Mouse Prkca Antibody (N-term) (Cat# 102-12404) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow). |
 | Mouse Prkca Antibody (N-term) (Cat# 102-12404) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
 | Mouse Prkca Antibody (N-term) (102-12404)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkca Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
MOUSE
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.